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<p><b>OBJECTIVE</b>To assess the feasibility of using saliva for Schistosomiasis japonica diagnosis.</p><p><b>METHODS</b>Schistosoma japonicum infected animal model was established. Pairs of saliva and serum samples from rabbits and chronic schistosomiasis patients were collected. Anti-schistosoma specific antibodies in saliva and serum were detected by indirect ELISA.</p><p><b>RESULTS</b>The specificities of antibody detection of rabbit saliva and serum were 93% (28/30) and 97% (29/30), respectively, and the sensitivities of antibody detection of rabbit serum and saliva were 100% (24/24) and 88% (21/24), respectively. A significant correlation (r = 0.5307, P = 0.0038 < 0.05) existed between anti-SEA IgG levels in serum and saliva. As with those in serum, anti-SEA IgG levels in saliva could reflect the state of infection and treatment. The sensitivity of antibody detection was 91% (29/32) for patient saliva samples and 100% (32/32) for their sera. 8 samples were positive in 140 normal saliva samples (i.e. 6% false positive rate) and 6 samples were positive in 156 normal serum samples (4% false positive rate). There was a significant correlation (r = 0.4227, P = 0.008 < 0.05) between specific antibodies in saliva and serum.</p><p><b>CONCLUSION</b>The detection of specific antibodies in saliva can be used as a non-invasive immunodiagnosis method of Schistosomiasis japonica.</p>
Subject(s)
Adolescent , Adult , Animals , Child , Female , Humans , Male , Middle Aged , Rabbits , Antibodies, Helminth , Enzyme-Linked Immunosorbent Assay , Immunoglobulin G , Saliva , Allergy and Immunology , Schistosoma japonicum , Allergy and Immunology , Schistosomiasis japonica , DiagnosisABSTRACT
Objective To explore the factors that influence the medical cost of advanced schistosomiasis patients in Anhui Province.Methods The medical records and other related data were collected from 13 pilot hospitals in Anhui Province.Single factor analysis and multiple regression methods on 264 cases of medical cost were used for the analysis.Results The results of single factor analysis showed that the history of ascites,ascites and concomitant diseases for advanced schistosomiasis medical cost had significant differences(P
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0.05),but the former was statistically higher than the latter from 2003-2005. Conclusions There is no obvious variation of the schistosome epidemicity at Jiangxin after resettlement but remaining arable land, while the epidemicity of Jiangzhou, after resettlement and converting cultivated land to forest or grassland, declines in some degree.
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Objective To explore the risk factors of cerebral schistosomiasis japonica. Methods A total of 37 confirmed cases,diagnosed during 1999-2004 in Anhui Province,were involved in the study as case group. Three controls for each case were selected from the general population either without schistosomiasis,with chronic schistosomiasis or with acute schistosomiasis. The relevant risk factors on the disease were analyzed. Results Four factors with labour intensity, physical, economical and nutritional conditions before illness between case group and normal control group, 7 factors including epilepsy history, hypersensitivity,intensity and times of infection between case group and acute schistosomiasis control group,10 factors including intensity of infection,times of infection and treatment between case group and chronic schistosomiasis control group, all showed statistical significance (p